ZFIN Image: Sousa et al., 2011, Fig. S2

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Figure Caption

Fig. S2

Expression of the reporter genes in the transgenic lines: Tg(osterix:mCherry) and Tg(osteocalcin:GFP) during early stages of caudal fin regeneration. (A-F) Whole-mount in situ hybridization for the reporter genes. (A-C) Detection of gfp mRNA in the Tg(osteocalcin:GFP) fins. (A) Uncut proximal portion of the fin before the fin bifurcation. (B) 24 hpa fin. (C) 48 hpa fin. Arrows in B and C indicate the absence of gfp mRNA at the blastema. (D-F) Detection of mCherry mRNA in the Tg(osterix-mCherry) fins. (D) Uncut distal portion of the fin. (E) 24 hpa fin. (F) 48 hpa fin. Scale bar: 100 μm in regenerating fins; 200 μm in uncut fins. Arrowheads indicate the amputation plane.

Acknowledgments

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