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Fig. S4
The effect of wdr18 knockdown on endodermal organs. (A, B) pdx1 was used as a marker for the pancreatic bud. In morphant embryos, the pancreas appeared on both sides of the body (B), while in control embryos it resided on the right side. (C, D) insulin (ins) was used as a differentiation marker for pancreatic β cells. Note that the development of β cells was not affected after wdr18 morpholino injection. (E, F) In situ hybridization of trypsin (try) revealing the exocrine pancreas at 4 dpf. In morphant embryos, the exocrine pancreas differentiated normally, but appeared in duplicated form. Dorsal views, anterior to the top.