ZFIN Image: Totong et al., 2011, Fig. S5

Image

Figure Caption

Fig. S5

tmem2 is broadly and maternally expressed. (A,B) Lateral views of wt embryos, dorsal up, anterior to the right. (A) In situ hybridization with a probe corresponding to bases 2446-3524 of the tmem2 coding sequence indicates ubiquitous expression at 13 som. (B) At 24 hpf, tmem2 is still broadly expressed throughout the embryo, with highest expression levels in the anterior. (C) RT-PCR using tmem2-specific primers and cDNA from wt embryos at the 2- to 8-cell stage indicates that tmem2 is maternally provided. The 1100 bp product in the first lane is a fragment of tmem2 cDNA. The -RT control lane shows no amplification when reverse transcriptase is excluded from the reverse transcription reaction. The lower bands in both lanes are primer dimers. Scale bar: 50 μm.

Figure Data

Expression / Labeling details

Acknowledgments

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