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Fig. 6

ID
ZDB-IMAGE-110914-31
Source
Figures for Shono et al., 2011
Image
Figure Caption

Fig. 6

Transient transgenic analysis of the gcm2 enhancer regions for HRCs specific to the skin surface.

(A, B) The chart on the right shows the percentage of embryos with Venus-positive cells on the skin surface at 24 hpf. (C–F) In situ hybridization with an atp6v1al probe in combination with immunohistochemistry using anti-GFP on the yolk sac of transient transgenic 4-day-old zebrafish. (C, D) A zebrafish expressing pTolfV that contains the gcm2 enhancer region that is 8 kb upstream. (E, F) A zebrafish expressing pTolfV that contains the gcm2 enhancer region that is 41 kb downstream. The gcm2 loci at -8249 to -8004 bp upstream and at +41277 to +41497 bp downstream were required for Venus expression in HRCs.

Acknowledgments
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