ZFIN Image: Johnson et al., 2011, Fig. S2

Image

Figure Caption

Fig. S2

The pharyngeal cartilage phenotype of vgll2a morphants can be partially rescued by mRNA encoding vgll2a. Examples of 5 dpf larvae stained with alcian blue in ventral views (A–C) and quantification of the number of pharyngeal cartilage elements visible for each condition examined (D). In wildtype (A) the total number of pharyngeal cartilage elements at 5 dpf is normally 18 (n = 9). Embryos injected at the 1 cell stage with 100 pg vgll2aΔMO mRNA in addition to 6 ng vgll2a ATG MO injected at the 2 to 4 cell stage (n = 9) averaged 4.7 alcian-positive pharyngeal cartilage elements, significantly more (Student′s T test, *: p < 0.05) than those injected withvgll2a ATG MO alone (n = 8), which averaged 1.3. Error bars represent standard error of the mean. Anterior is to the left in A–C.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 357(1), Johnson, C.W., Hernandez-Lagunas, L., Feng, W., Melvin, V.S., Williams, T., and Artinger, K.B., Vgll2a is required for neural crest cell survival during zebrafish craniofacial development, 269-81, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.