ZFIN Image: Cheung et al., 2011, Fig. 15

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Figure Caption

Fig. 15

Effect of 2-APB and ryanodine on the organization of the trunk musculature and the formation of sarcomeres. Embryos were either (A) untreated (controls) or else (B-F) treated from 17 hpf to 20 hpf with: (B) 1% DMSO (DMSO control); (C,D) 2-APB at (C) 25 μM or (D) 50 μM; or (E,F) ryanodine at (E) 10 μM or (F) 50 μM. Embryos were then fixed and the myosin and F-actin labeled with (Ai-Fi) the F59 myosin heavy chain antibody and (Aii-Fii) rhodamine-phalloidin, respectively. (Aiii-Fiii) The merged myosin and F-actin images, with the overlapping regions shown in yellow. (Ai*-Aiii* to Fi*-Fiii*) Higher magnification views of (Ai-Aiii - Fi-Fiii), respectively, showing the level of sarcomeric banding in the slow muscle fibers in the untreated and drug treated embryos. Scale bars are (Ai-Aiii to Fi-Fiii) 25 μm and (Ai*-Aiii* to Fi*-Fiii*) 5 μm.

Acknowledgments

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