ZFIN Image: Paridaen et al., 2011, Fig. S5

Image

Figure Caption

Fig. S5

Gene expression changes upon Gnl2 LOF. (A and B) The earliest morphologically apparent phenotype of gnl2 mutants at 22 hpf, consisting of reduced forebrain (arrow and dashed lines), a thinner mid/hindbrain boundary (arrowhead) and smaller eyes. (C) Differentially expressed genes upon loss of Gnl2 at 22 hpf, categorized according to Gene Ontology (GO) function. A GO function was found for 23 out of the 44 total downregulated genes and 64 out of 96 total upregulated genes. (D–I) Confirmation of reduced expression of gnl2 (D and E; n = 9) and increased expression of the direct p53 transcriptional targets mdm2 (F and G; n = 6) and ccng1 (H and I; n = 7) in gnl2 mutants (E, G and I) as shown by in situ hybridization. All images are lateral view, anterior to the left. Scalebar 125 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 355(2), Paridaen, J.T., Janson, E., Utami, K.H., Pereboom, T.C., Essers, P.B., van Rooijen, C., Zivkovic, D., and Macinnes, A.W., The nucleolar GTP-binding proteins Gnl2 and nucleostemin are required for retinal neurogenesis in developing zebrafish, 286-301, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.