ZFIN Image: Kikuchi et al., 2011, Fig. 6

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Figure Caption

Fig. 6

Epicardial cells contribute vascular support cells during heart regeneration. (A) Schematic of the experimental design. (B,C) Lineage-labeled EGFP⁺ cells (arrows, green) did not colocalize with Myosin heavy chain (MHC; B, red) or Mef2 (C, red) in 30 days post-amputation (dpa) regenerates of the larvally labeled animals (brackets; n=20). (D,E) EGFP⁺ cells (arrowheads) surrounds a vessel within the larvally labeled regenerate. Asterisk marks vascular lumen. (F) Schematic of the experimental design. (G,H) Lineage-labeled EGFP⁺ cells (arrows) did not colocalize with MHC (G) or Mef2 (H) in 30 dpa regenerates of the adult-labeled animals (brackets; n=6). (I,J) EGFP⁺ cells (arrowheads) surround a vessel within the adult-labeled regenerate. Asterisk marks vascular lumen. An antibody was used to detect EGFP in these experiments. Scale bars: 50 μm for B,C,G,H; 10 μm for D,E,I,J.

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