ZFIN Image: Matsui et al., 2011, Fig. S3

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Figure Caption

Fig. S3

cnpy1 function is essential for DFC clustering. (A, B) sox32 expression in uninjected (A) or cnpy1-MO-injected (B) embryos. Scale bar: 200 μm. (C) Percentages of normal or broken-up DFCs were scored using the no tail expression pattern in uninjected (n = 84), cnpy1-MO (n = 55), DFC^control-MO (n = 64), DFC^cnpy1-MO (n = 48), yolk^control-MO (n = 50) or yolk^cnpy1-MO (n = 66) embryos. Statistically significant (P < 0.05) differences could be seen in uninjected versus cnpy1-MO (P = 2.76 x 10^-9) and DFC^control-MO versus DFC^cnpy1-MO (P = 6.1 x 10^-5), while no difference was seen in uninjected versus DFC^control-MO (P = 0.403), uninjected versus yolk^control-MO (P = 0.361), DFC^control-MO versus yolk^control-MO (P = 1.000) or yolkcontrol-MO versus yolkcnpy1-MO (P = 0.314). (D-G) no tail expression in uninjected (D), cnpy1-MO-injected (E), DFC^control-MO (F) or DFC^cnpy1-MO (G) embryos. Dorsal view, anterior to the top. Scale bar: 200 μm. (A′-D′) Higher-magnification images highlight DFCs. The white dotted lines mark the boundary between DFCs and the blastoderm margin.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA