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Fig. 4
Immunohistochemical analyses of Sycp3 in the its, isa, and imo mutant testes. Fresh-frozen testes sections from wild-type (A), its (B), isa (C), and imo (D) zebrafish were stained with anti-Sycp3 antibodies (white in upper panels, green in lower panels), and counterstained with TOPRO-3 (magenta). Merged images with counterstaining are shown in the bottom panels. Sycp3 is a structural component of the lateral element of the synaptonemal complex, and is an established meiotic prophase I marker. Note the higher incidence of stained cells in the mutant testis compared with wild-type, indicating that a higher number of spermatocytes has accumulated during meiotic prophase I. Scale bar = 100 μm.