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Fig. S3
Analysis of reporter gene expression in other tissues than the pectoral fins: the zfprrx1b1CNE:EGFP lines. A–E: zfprrx1b1(cFos):EGFP line#5. Expression in this transgenic line is very pectoral fin-specific until at least 30 hours postfertilization (hpf; Fig. 6 and C here). F–J: zfprrx1b1(cFos):EGFP line#8. Apart from the expression in the developing pectoral fins from 27 hpf onward (marked here by arrowheads in H–J), enhanced green fluorescent protein (EGFP) signal is also observed in some cells of the spinal cord from 18 to 48 hpf (arrows in F–I). Because this expression site is not observed in the other transgenic line generated by injection of the same DNA construct (zfprrx1b1(cFos):EGFP line#5), it is most probably due to a positional effect. Similar to the expression observed in the zfprrx1aCNE:EGFP lines EGFP signal is also observed in scattered cells surrounding the somatic muscles at 48 and 72 hpf (double arrows in D, E and I, J), suggesting these represent true expression sites driven by the orthologous enhancers zfprrx1a and zfprrx1b1 CNEs. Again, nonspecific expression in the yolk extension is observed (A–J).