Fig. 2
GFP expression in stable transgenic lines consistent with tbx16 expression in wild type embryos.
(a) At 12 hpf strong expression is seen in the polster (arrowhead), and the presomitic mesoderm (p). Expression persists in mesoderm that has formed somites (s). (b) At 24 hpf GFP expression is seen in the hatching gland (arrowhead) and in posterior somites, while it has faded from the earliest formed somites. (c) At 48 hpf expression remains in the hatching gland that has formed from the polster (arrowhead) and persists in the posterior somites. (d) The same embryo as in (c). GFP expression is observed throughout the notochord (n) highlighting cell extremities. Lateral views of embryos, anterior is left and dorsal is up. Scale bars in a–c = 250 μm, in d = 100 μm.