Fig. 7
Increased cell proliferation is detected in intestines of klf4a morphants.
(A) Images of p-Histone H3-stained cells in intestines of 72-hpf wild type and klf4a-MO1 and klf4a-MO2-injected embryos are shown. Comparison of p-Histone H3-stained cell percentages in intestines of wild type and klf4a morphants is shown. Arrows indicate p-Histone H3-stained cells. Error bars indicate the standard error. Student′s t-test was conducted to compare klf4a morphants with wild type embryos. (B) Images of BrdU-labeled cells in the foregut, midgut, and hindgut of 96-hpf klf4a-5mmMO2-injected and klf4a-MO1 and klf4a-MO2-injected embryos are shown. Comparison of BrdU-labeled cell percentages in intestines and in respective foregut, midgut and hindgut of klf4a-5mmMO2-injected and klf4a morphants are shown in panel A. Arrows indicate BrdU-labeled cells. Error bars indicate the standard error. Student′s t-test was conducted to compare klf4a morphants with klf4a-5mmMO2-injected embryos.