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Fig. 3
RNA whole mount in situ hybridization of 3 dpf embryos with dat probe didn′t show significant loss of DA neurons in morphant.
E to H, L and M, enlargement of the area of DA neurons in A to D, J, and K, respectively. The numbers on bottom right corner showed number of the embryos with a certain phenotype/number of total embryos in that group. The patterns of DA neurons in most embryos were normal, while some were disorganized. I, the quantitative result of dat positive neurons in the diencephalon. There is no significant DA neuron loss in morphant group. n = 20 in each group, P>>0.05 in all comparisons.
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