ZFIN Image: Sedletcaia et al., 2011, Fig. 1

Image

Figure Caption

Fig. 1

Both tbx2a and tbx2b are expressed in the primitive heart tube and transcripts become restricted to the atrioventricular canal (AVC) by 2 days postfertilization (dpf). A–D,G–I: Shown are representative embryos (n > 30 for each) analyzed by in situ hybridization to detect transcripts for tbx2a (A,B,G), tbx2b (C,D,H), or the AVC marker bmp4 (I). A–D: In addition to strong staining in the head, transcripts are readily detected in the primitive heart tube at 36 hpf (A,C, between the dashed lines) and subsequently in the AVC at 3 dpf (B,D, arrowheads marking the cushion tissue on either side of the AVC). E,F: Also shown for purpose of orientation are similarly staged myl7:gfp transgenic embryos that show the position of the heart tube at 36 hpf (E) and the AVC at 3 dpf (F). G–I: The restriction to the AVC can be seen starting already at 2 dpf, compared with bmp4. Control sense strand probes failed to generate any detectable signal under the identical conditions (not shown).

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.