ZFIN Image: Bruses, 2011, Fig. 6

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Figure Caption

Fig. 6

MiP motor axon targeting is unaffected in N-cadherin mutant embryos. Wild-type (A) and cdh2^hi3644Tg mutant (B) embryos were fixed at 27 hpf, immunolabeled with znp1 antibodies, and observed via confocal microscopy. Arrowheads point to the presumptive MiP axon in the dorsal myotome. The axons appear to have followed a distorted pattern of migration. C,D: Single MiP motor neurons labeled by mosaic expression of pren-EGFP using a mix of a plasmid expressing Gal4 under the mnx1 promoter and a plasmid expressing pren-EGFP under a 14X-UAS element. In all cases, the motor axons reached the horizontal myoseptum and extended a dorsal branch. Wild type, n = 6; cdh2^hi3644Tg mutant, n = 6. Rostral is to the left and dorsal is to the top. Scale bars = 10 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Neurol.