ZFIN Image: Chou et al., 2011, Fig. 4

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Figure Caption

Fig. 4

Subcellular localization patterns of wild-type or phosphomutant fascin 2b proteins in live COS-7 cells that coexpress espin.

A cell (A–D) coexpressing GFP-WT fascin 2b and mCherry (mCh)-espin displays colocalization of these proteins in filopodia, which are detected as yellow in image overlays (C,D). Images of cells that coexpress mCherry-espin and either GFP-S38D fascin 2b (E–H) or GFP-S38E fascin 2b (I–L) reveal espin-laden protrusions (red) that lack the fascin 2b phosphomutant proteins (G,H,K,L). Micrographs of a cell that expresses both mCherry-espin and GFP-S38A fascin 2b (M–P) show that both proteins reside in filopodia (yellow) (O,P). Scale bars are 5 μm.

Acknowledgments

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