ZFIN Image: Ikenaga et al., 2011, Fig. 2

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Figure Caption

Fig. 2

Pax8 expression is identical to RFP expression. Transcripts were detected using in situ hybidization with either pax8 (A,B,G,H) or RFP (C–F) probes in 24-hpf (A,C,E,G,H) or 48-hpf (B,D,F) embryos. Expression pattern, at 24 hpf, of pax8 in wild type (pax8^+/+; A) was the same as that of RFP in pax8^+/m (C) and pax8^m/m (E). Signal was detected in the mhb region of the embryos. RFP showed no signal in wild-type samples (data not shown). At 48 hpf, the staining pattern of pax8 in wild type (B) resembled that of RFP expression in pax8^+/m (D) and pax8^m/m (F) embryos. Pax8^+/m embryos showed positive staining also with the pax8 probe (G). Pax8 transcripts were also detected, at a much lower intensity, in pax8^m/m embryos (H). Scale bars = 100 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Neurol.