ZFIN Image: Ikenaga et al., 2011, Fig. 9

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Figure Caption

Fig. 9

Spinal neurons in pax2a/pax8 double mutant. A,B: RFP fluorescence of a pax8^+/m embryo (A) and a double-mutant embryo (B) under the same optical condition. The RFP signal is reduced in the double mutant. C,D: The RFP signal in the spinal cord of a pax8^+/m embryo (C) and a double mutant embryo (D) near the fifteenth body segment. 3D images were constructed from confocal slices. E–H: Stochastic labeling by GFP (green) identified a neuron with an ipsilateral descending axon in the double mutant. E is a 3D reconstruction. F (RFP), G (GFP), and H (merged) are single-plane images. I,J: The spinal cord of a pax8^+/m larva obtained from crossing with the Alx-GFP transgenic line. The image is from an embryo at 4 dpf. I is a 3D reconstruction and J is a single confocal plane. Note that RFP and GFP do not overlap. Magenta-green images are provided in Supporting Information Figure 6. Scale bars = 1 mm in B (applies to A,B); 20 μm in D (applies to C,D); 50 μm in E; 20 μm in J (applies to I,J).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Comp. Neurol.