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Fig. s5
Phenotypic analyses of Slit/Robo morphants or mutants. (A) Phenotypic analysis of slit2, slit3, robo1 and robo4 morphants at 48 hpf. slit2, robo1 and robo4 morphants displayed circulation defects (gata1:dsRed) but normal vascular patterning (kdrl:GFP). Pericardial edema is indicated by a black arrow. slit3 knockdown results in defects in the intersomitic vessels and dorsal longitudinal anastomotic vessels, including broken or missing vessels (white arrows); however, pericardial edema does not occur in slit3 morphants. Whereas circulation is defective in the intersomitic vessels and dorsal longitudinal anastomotic vessels, circulation appears normal in the axial vessels. h, head; ht, heart; da, dorsal aorta; pcv, posterior cardinal vein; isv, intersomitic vessel; dlav, dorsal longitudinal anastomotic vessel. (B) robo2-/- (astray) mutants do not have obvious circulation defects or edema at 72 hpf. The pou4f3:GFP transgene marks the retinal ganglion cell axons (arrow), the migration of which is defective in astray mutants. Scale bars: 100 μm.