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Fig. s4
Slit/Robo signaling does not appear to affect vessel sprouting and miR-218 does not control vascular integrity. (A) Images of the sprouting of the intersomitic vessels (isv) in morphants by confocal microscopy. Tg(kdrl:GFP) labels the endothelial cells. The somite boundaries are indicated by F59 staining (red). vegfa morphants had severe sprouting defects, but sprouting appeared unaffected in other morphants. Scale bar: 100 μm. (B) Sprouting phenotypes were quantified by determining the percentage of the intersomitic vessels that were present and the percentage of intersomitic vessels that crossed the myoseptum. The total numbers of embryos analyzed were as follows: control, n=29; slit2 MO, n=19; miR-218 MO1, n=14; robo1 MO, n=13; robo4 MO, n=17; vegfa MO, n=20. Data are mean + s.e.m. (C) Vascular integrity was assessed by confocal microscopy of transverse Vibratome sections of embryos at 48 hpf. The axial vasculature was lumenized in miR-218 morphants as determined by the presence of gata1:dsRed+ erythrocytes within the axial vessels. Scale bar: 25 μm.