ZFIN Image: Waxman et al., 2011, Fig. s1

Image

Figure Caption

Fig. s1

RAR-specific pharmacological reagents affect expression of Tg(12XRARE-ef1a:gfp), while pharmacological reagents specific for other nuclear hormone receptors do not. (A) Untreated control embryo. (B) Ro41-5253, a RARα-specific antagonist, inhibits reporter expression. (C) AM580, a RARα-specific agonist, activates ectopic reporter expression. Arrows (ventral eye) and arrowheads (anterior spinal cord) in B and C indicate where restricted expression is normally found in an untreated embryo (A). (D) The pan-RXR agonist methoprene acid (Biomol.com) does not affect reporter expression. Agonists of (E) PPARα (GW7647), (F) PPARδ (GW0742), and (G) PPARγ (GW1929) do not activate the reporter. The pan-PPAR antagonist LY171883(H) and the PPARα specific antagonist GW6471 (I) do not inhibit reporter expression. All PPAR-related reagents were purchased from Sigma. All images are lateral views, with anterior up and dorsal to the right

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 352(1), Waxman, J.S., and Yelon, D., Zebrafish retinoic acid receptors function as context-dependent transcriptional activators, 128-140, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.