ZFIN Image: Waxman et al., 2011, Fig. 2

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Figure Caption

Fig. 2

Zebrafish RARE transgenic reporter lines require and are responsive to RA signaling. (A) Untreated Tg(12XRARE-ef1a:gfp) embryo. (B) Treatment of Tg(12XRARE-ef1a:gfp) embryos with 0.2 μM RA induces reporter expression. (C, D) Tg(12XRARE-ef1a:gfp) and Tg(12XRARE-tk:gfp) embryos respond to treatment with RA for 6 h beginning at 24 hpf (C) or 30 hpf (D). Untreated sibling embryos (left) exhibit expression in the anterior spinal cord (arrowhead), the ventral eye, and pronephros. Treatment with 0.2 μM RA (center) causes a more modest response than does treatment with 0.5 μM RA (right). Increased fluorescence is induced in the anterior brain/eye (upper arrow), midbrain and spinal cord (arrowheads) and pronephros (lower arrow). (E, H) Untreated Tg(12XRARE-ef1a:gfp) (E) and Tg(12XRARE-tk:gfp) (H) embryos, respectively. Treatment with RA signaling antagonists BMS (F, I) and DEAB (G, J) inhibit expression of Tg(12XRARE-ef1a:gfp) and Tg(12XRARE-tk:gfp). Embryos were treated with RA signaling antagonists for 6 h beginning at 24 hpf (E–G) or 30 hpf (H–J). All images are lateral views, with anterior up and dorsal to the right.

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Reprinted from Developmental Biology, 352(1), Waxman, J.S., and Yelon, D., Zebrafish retinoic acid receptors function as context-dependent transcriptional activators, 128-140, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.