ZFIN Image: Lupo et al., 2011, Fig. 1

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Fig. 1

Inhibition of RAR signaling causes coloboma in zebrafish. (A) Coloboma (arrowheads) in embryos treated with 2.5–5 μM AGN from 3 to 6 s. (Left) Lateral view of 48-hpf phenylthiourea-treated embryos. (Center) Lateral (Center Left) and ventral (Center Right) views of 60-hpf eyes. (Right) Sections of 60-hpf eyes showing the lack of retinal pigmented epithelium (triangles) and a shortening of the retina ventral to the optic nerve head (arrows) in the AGN-treated embryo. (B, Left) Immunostaining with an anti-acetylated (Ac.) tubulin antibody at 48–50 hpf, showing abnormal retinal axon trajectories in the AGN-treated embryo (arrowheads), besides the normal contralateral projection (triangles). (Center) fli1a:EGFP transgenic embryos at 48 hpf. The AGN-treated embryo exhibits disrupted vascularization (triangles). (Right) sox10:EGFP transgenic embryos at 32–36 hpf. The AGN-treated embryo shows less POM cells within the choroid fissure (triangles).

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA