ZFIN Image: Angotzi et al., 2011, Fig. 3

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Figure Caption

Fig. 3

Morpholino knock down effects for pit1, prl, tshβ and gh assessed by a ΔΔC_t method to determine relative gene expression from qPCR data, using ef1a as an endogenous reference gene. In an MO knockdown experiment (MO), ΔC_t expression is normalized to a corresponding control sample (Co). Percent knockdown was calculated by subtracting the normalized ΔΔC_t expression from 1 (defined by the level of expression for control sample) and multiplying by 100. Error bars indicate SDM for δC_t expression in morpholino and control samples. Asterisks indicate statistically significant differences (P < 0.05). NS, not statistically significant. prop1 MO significantly downregulates pit1 (64%), prl (47%) and gh (79%) expression at early but not at late stages.

Acknowledgments

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