Fig. 4
Pit1 expression patterns of controls (A–C) and morphants (D–F). At 24 hpf, pit1 expression in morphants was mostly restricted to the medial region of the adenohypophyseal placodes (D/E) whereas in most controls it expanded laterally and posterior to generate an arch-like signal (A) or in few cases a globular expression domain (B). Defective pit1 expression pattern in the adenohypophysis of morphants was still observed during subsequent development (72 hpf; F vs. C). Gene names are indicated in the lower left-hand corner of each panel. Age (in hpf) and (treatments) in the upper right-hand corner. Scale bar for (A/B; D/E) = 100 μm; (C/F) = 25 μm.