ZFIN Image: Knopf et al., 2011, Fig. 2

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Figure Caption

Fig. 2

Stump Osteoblasts Induce Markers of Osteoblast Progenitors and Lose Their Differentiated Morphology

(A) Whole-mount in situ hybridization against runx2b in wild-type fins at 0 hpa and 1 dpa (n = 5 fish each). Scale bar, 200 μm.

(B) Whole-mount in situ hybridization against gfp in runx2:GFP fins at 0 hpa and 1 dpa (n = 5 fish each). Scale bar, 200 μm.

(C) The extracellular matrix protein Tenascin is not expressed in osteoblasts on the inner and outer surface of the bone at 0 hpa (n = 2 fish, 19/21 sections) but is detectable at 1 dpa in distal stump osteoblasts on both sides of the bone as well as in intraray fibroblast-like cells (n = 5 fish, 48/48 sections). Stump osteoblasts adopt a rounded shape at 1 dpa and are identified by the presence of Zns 5, which is primarily localized at the cell surface. Scale bars, 10 μm.

(D) Ultrastructural changes of osteoblasts after amputation. Toluidine blue staining and corresponding transmission electron micrographs of uncut and 1 dpa fins. Some cells can be detected covering the stump bone (black arrowhead in Toluidine blue staining). Nuc, nucleus; ECM, extracellular matrix; Epid, epidermis; BM, basal membrane. Scale bars, 20 μm (Toluidine blue stainings) and 2 μm (TEMs).

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Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Cell, 20(5), Knopf, F., Hammond, C., Chekuru, A., Kurth, T., Hans, S., Weber, C.W., Mahatma, G., Fisher, S., Brand, M., Schulte-Merker, S., and Weidinger, G., Bone Regenerates via Dedifferentiation of Osteoblasts in the Zebrafish Fin, 713-724, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell