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Fig. 7 The absence of Bmp signaling induces an expansion of the bulbus arteriosus at the expense of the ventricle. (A-C) Ventral view of 72 hpf zebrafish treated with dorsomorphin (B,C) or DMSO carrier (A) from 24 to 72 hpf. (D) The bulbus arteriosus can be detected at 52 hpf as shown by anti-Eln2 labeling. A, atrium (S46); V, ventricle (MF20). (E,F) Ventral view of 48 hpf embryos. Dorsomorphin treatment (F) from 24 to 48 hpf leads to smaller ventricles (red) at 48 hpf. (G,H) Sagittal sections of embryos with dorsal to the top. Dorsomorphin treatment from 24 to 30 hpf (H) results in a significant loss of phosphorylation of Smad1/5/8 (green nuclei) at the arterial pole (arrow). Scale bars: 5 μm.