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Figure Caption
Fig. 5 Isl1 is expressed by cells that are being incorporated into the arterial pole up to 48 hpf. Sagittal sections of Tg(cmlc2::GFP) zebrafish, cranial to the top, dorsal to the left. (A-C′) Isl1 (green) is expressed in cells adjacent to and within the arterial pole (arrows) at 24, 36 and 48 hpf during addition of the second heart field myocardial cells to the heart tube (GFP, red). (D,D′) By 72 hpf, Isl1 is absent from the myocardium and is expressed in the cranial-most portion of the smooth muscle of the bulbus arteriosus. A, atrium; V, ventricle; Y, yolk. Scale bar: 5 μm.
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