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Fig. S2 Apoptosis is observed in 6 and 12 hours postfertilization (hpf)lzap morphants. TUNEL (terminal deoxynucleotidyl transferase–mediated deoxyuridinetriphosphate nick end-labeling) staining was used to identify apoptotic cells (indicated by arrows) at 6 and 12 hpf. Apoptosis was observed in scattered cells within the embryo after lzap depletion, while no apoptosis is detected in uninjected controls. At 12 hpf, apoptotic cells were observed primarily in rostral locations in control embryos, while apoptotic cells were more widely dispersed in lzap morphants.
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