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Fig. S2 The thsd7a expression levels increase during embryogenesis. Real-time quantification polymerase chain reaction (RT-qPCR) was performed on zebrafish embryos at different developmental stages. The thsd7a expression level was first normalized by beta-actin expression and then by the level at stage 2.25 hours postfertilization (hpf), and was plotted as mean ± SEM. A small amount of thsd7a transcript was detected at 2.25 hpf. Zygotic thsd7a was up-regulated at 15 hpf and persistently increased during the examined developmental period.
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