ZFIN Image: Fujita et al., 2011, Fig. S9

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Figure Caption

Fig. S9

PI3K signaling is required for HUVEC migration in vitro and for PHBC migration in vivo. (A) SDF-1α-induced dose-dependent migration of HUVECs after a 5 hour incubation, measuring the number of transwell-migrated cells. (B) HUVEC transwell migration in response to 100 ng/ml SDF-1α (column 2) and after pretreatment with either 1 mM AMD3100 (column 4) or 10 mM LY294002 (column 6). Shown (A,B) are the mean (with s.e.m.) obtained by measuring eight randomly selected fields of transwell-migrated cells. (C,D) Confocal images of PHBCs and BA in the hindbrain of 32 hpf Tg(fli1a:EGFP)^y1 animals treated from 24-32 hpf with either control DMSO carrier (C) or the PI3K inhibitor LY294002 (D). Dorsal view, rostral to the left. Scale bar: 50 μm.

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