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Fig. S4
VEGF signaling is required for hindbrain vascular patterning. (A,B) Confocal images of the PHBC, BA and CtA in the hindbrain of 30 hpf Tg(kdrl:mCherry-CAAX)y171 animals treated from 24-32 hpf with either control DMSO carrier (A) or the VEGFR inhibitor SU5416 (B). PHBC sprouting defects are present and BA fails to form (arrowheads) in embryos treated with SU5416 after PHBC assembly. PHBC size (yellow brackets) and CtA sprouting (arrows) are also affected in SU5416-treated embryos. Scale bar: 50 μm.
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