ZFIN Image: Kikuchi et al., 2011, Fig. 3

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Figure Caption

Fig. 3

Transgenic Inhibition of RA Signaling Blocks Cardiomyocyte Proliferation during Regeneration

(A) Assessment of Mef2⁺PCNA⁺ cells (arrows) in wild-type (wt) and hsp70:dn-zrar transgenic fish at 7 dpa, after a single heat-shock at 6 dpa. Maximum projection images of 10 μm Z stacks are shown. Insets, high-magnification images of the rectangle. Arrowheads, proliferating epicardial cells; brackets, injury site. Scale bar represents 100 μm (A and C).

(B) Quantification of CM proliferation in wt and hsp70:dn-zrar transgenic fish at 7 dpa. Data are mean ± standard error of mean (SEM) from 6 animals each (3097 wt and 2482 transgenic CMs analyzed). ^*p < 3 × 10^-5, Student′s t test.

(C) Assessment of Mef2/PCNA double-positive cells (arrows) in wt and hsp70:cyp26a1 transgenic fish at 7 dpa, after a single heat-shock at 6 dpa. Maximum projection images of 10 μm Z stacks are shown.

(D) Quantification of CM proliferation in wt and hsp70:cyp26a1 transgenic fish at 7 dpa. Data are mean ± SEM from 4 wt and 6 transgenic animals (3888 wt and 4760 transgenic CMs analyzed). ^*p < 2 × 10^-4, Student′s t test.

Acknowledgments

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Reprinted from Developmental Cell, 20(3), Kikuchi, K., Holdway, J.E., Major, R.J., Blum, N., Dahn, R.D., Begemann, G., and Poss, K.D., Retinoic Acid production by endocardium and epicardium is an injury response essential for zebrafish heart regeneration, 397-404, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell