Fig. 3

Fig. 3 Ectopic expression of truncated forms of Tsc2 increases TORC1 activity and causes morphological defects in the brain. (A–D) Lateral view of uninjected wild-type control (A), tsc2 wild-type RNA-injected (B), tsc2^vu242 RNA-injected (C) and tsc2/NruI-truncated RNA-injected (D) embryos at the one-somite stage (10.3 hpf). Embryos were labeled with an antibody to phospho-S6 (A–H). (E–H) Dorsal view of A–D. (I–L) Lateral views (anterior to the left) of control (I), tsc2 RNA-injected (J), tsc2^vu242 RNA-injected (K) and tsc2/NruI-truncated RNA-injected (L) embryos at 27 hpf. Solid lines mark the hindbrain boundary (I–L). (M) Schematic of constructs used for the production of synthetic RNAs for injection experiments. Scale bars: 100 μm.