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Fig. S3 Patterning of the otic vesicle following misexpression of sox3. Expression of dlx3b, otx1, pax5, and neurod in control embryos (A, C, E, G) and in embryos transiently transfected with hs:sox3 (B, D, F, H). Embryos were heat shocked for 30 min at 39 °C beginning at 11.5 hpf, after which embryos were maintained at 33 °C. These conditions yield maximal expression of the heat shock vector for 90 min, followed by sustained lower level misexpression until fixation at 26 hpf.
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Reprinted from Developmental Biology, 351(1), Padanad, M.S., and Riley, B.B., Pax2/8 proteins coordinate sequential induction of otic and epibranchial placodes through differential regulation of foxi1, sox3 and fgf24, 90-98, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.