ZFIN Image: Miyasaka et al., 2011, Fig. S4

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Figure Caption

Fig. S4 (A) As a combination of gain-of-function and rescue experiments, we soaked zebrafish embryos in a saline solution containing 0.2μM of RA from 24 to 60 hpf. Approcimately 75% of RA-treated embryos displayed severe edema in the epicardial sacs (red arrowheads). (B) When a duplex of miR-143 was injected, RA-induced edema was reduced to only mild to weak edema. (C) Occurrence of the edematous phenotype in the heart. Injection of the miR-143 duplez resulted in the edematous phenotype in all embryos, with severe epicardial edema in more than 70% of cases. When the RA treatment and the injection of the duplex were combined, this phenotype became milder.

Acknowledgments

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Reprinted from Mechanisms of Development, 128(1-2), Miyasaka, K.Y., Kida, Y.S., Banjo, T., Ueki, Y., Nagayama, K., Matsumoto, T., Sato, M., and Ogura, T., Heartbeat regulates cardiogenesis by suppressing retinoic acid signaling via expression of miR-143, 18-28, Copyright (2011) with permission from Elsevier. Full text @ Mech. Dev.