ZFIN Image: Yoo et al., 2010, Fig. 5

Image

Figure Caption

Fig. 5 PI(3)K Regulates Anteroposterior Polarity of F-Actin Dynamics

(A) Stable F-actin (GFP-UtrCH) is localized at the tail while dynamic F-actin (Lifeact-Ruby subtracted by GFP-UtrCH) is localized at the front (Movie S9).

(B) In control, GFP-UtrCH labels the tail.

(C) PI(3)K inhibition by LY294002 disturbs tail localization of stable F-actin (Movie S11A).

(D) Myosin ATPase and Rho kinase inhibition disturbs tail localization of stable F-actin (Movie S11C).

(E) Constitutively active RhoQ63L induces cell rounding and localization of stable F-actin all over the membrane (top panel). PI(3)K inhibition does not relieve constitutively active Rho-mediated effects on cell rounding or localization of stable F-actin (lower panel) (Movie S12A). Images are representative of more than five time-lapse movies from experiments repeated on at least two separate dates.

Scale bars = 10 μm.

Acknowledgments

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Reprinted from Developmental Cell, 18(2), Yoo, S.K., Deng, Q., Cavnar, P.J., Wu, Y.I., Hahn, K.M., and Huttenlocher, A., Differential Regulation of Protrusion and Polarity by PI(3)K during Neutrophil Motility in Live Zebrafish, 226-236, Copyright (2010) with permission from Elsevier. Full text @ Dev. Cell