ZFIN Image: Barrios et al., 2003, Fig. S4

Image

Figure Caption

Fig. S4 EphA4 Is Removed from the Basal Cell Membrane during Somite Boundary Formation
(A–F and A′–F′) (A–F) Confocal fluorescence and DIC overlays and (A′–F′) DIC images of EphA4eGFP-expressing cells (green) in the paraxial mesoderm of a (A–C) wild-type and a (D–F) fss^-/- embryo during somite furrow formation. The localization of EphA4eGFP is monitored through time (indicated in minutes at the bottom left). The arrowheads point at the interface between EphA4-expressing cells and nonexpressing cells, where the furrow of de-adhesion is forming and EphA4eGFP protein is being cleared. These observations suggest that Eph/Ephrin signaling may be terminated after boundary formation by the removal of receptor or receptor/ligand signaling complexes in both wild-type intersomitic boundaries and boundaries restored in fss^-/- embryos. The scale bar represents 10 μm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Curr. Biol.