ZFIN Image: Ravanelli et al., 2011, Fig. S5

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Figure Caption

Fig. S5 Anti-Cobl staining levels correlate with severity of morphant phenotypes.

Control and cobl morphant embryos of both classes of severity were stained with anti-zfCobl and anti-acetylated tubulin, and assessed for correlations between phenotypic severity and Cobl levels. (A) Q-PCR results showing average levels of WT cobl mRNA in controls (n = 9) vs. cobl-MOa morphants (n = 30) at 24 hpf. p value = 0.0004*. (B) Control embryos show WT levels of anti-Cobl staining in the pronephric duct at 36 hpf by pixel intensity. (C-D) Morphant embryos reveal reduced anti-Cobl staining and cilia length. (E) Bar graph depicting the correlation between phenotypic class and levels of Cobl by antibody staining. p values: control/class 1 = 0.0676, control/class 2 = 0.0052*, class 1/class 2 = 0.1941. Scale bar represents 10 μm.

Acknowledgments

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Reprinted from Developmental Biology, 350(1), Ravanelli, A.M., and Klingensmith, J., The Actin Nucleator Cordon-bleu is Required for Development of Motile Cilia in Zebrafish, 101-111, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.