Fig. S6

Fig. S6 Shield-stage lens mosaics. Cartoon schematic of shield-stage transplants. Donor embryos are injected with Alexa Fluor 488 dextran (10 kDa) and grown to the shield stage. Animal pole view of shield-stage host embryos (shield is located at 12 o′clock in the cartoon), indicating the approximate location of the lens-fated region of the embryo into which donor cells were transplanted. Embryos were grown to 34 hpf and screened for percent lens composition and classed into “Low” (< 30% donor cells), “Medium” (30–70% donor cells) or “High” (> 70% donor cells) groups. Embryos with any retinal contamination were discarded. The genotype of donor and host embryos was determined at 5 dpf based on phenotype, and only those involving severe mutants were used for subsequent analyses.