|
Fig. S3 Early Notch-responsive progenitors contribute to principal islet α- and δ-cells. (A-H) Confocal images of single optic sections of micro-dissected pancreata from GIFM larvae at 5 dpf. Fish are also transgenic for either (A-D) gcga:eGFP (marking α-cells with GFP) or (E-H) SST2:eGFP (marking δ-cells with GFP). Larvae were treated either with 4OHT (4OHT treated) or vehicle alone (-ve control) at 12-36 hpf. (A,E) Tissue-specific markers in GFP and nuclei in DAPI (blue). (B,F) Lineage traced cells with red labeled nuclei. (C,G) Red and green images merged with bright field. Cells positive for both tissue-specific transgene and lineage labeling appear yellow and examples are indicated with black arrows. (D,H) Without treatment with 4OHT (negative or -ve control) no endocrine cells are labeled.