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Figure Caption
Fig. 5 RA downregulates the Hh receptor ptc1 in endodermal explants. (A) qPCR of ptc1 and smo expression levels in RA-treated zebrafish endodermal explants (mean ± s.e.m.). Asterisk indicates a significant change (at least 2 s.d.) compared with control. (B,C) Tg(ins:GFP) endodermal explants treated with DMSO (B) or 1 μM RA (C). To create the endoderm explants, sox32 mRNA was injected into Tg(ins:GFP) embryos at the 1-cell stage (see text).
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