|
Fig. 3 rumbahkz1 and sambahkz2 mutations cause different cellular defects in definitive hematopoietic cells. (A-H) Double staining of cmyb RNA and phospho histone 3 (pH3) protein in the CHT of 4 dpf wild-type (WT) zebrafish siblings (A-D) and sambahkz2-/- mutants (E-H). D and H show bright-field images with fluorescent staining overlaid. (I-P) Triple staining of Cd41-GFP, BrdU and phospho histone 3 (pH3) of 3 dpf wild-type siblings (I-L) and rumbahkz1-/- mutants (M-P). Arrows indicate Cd41-GFP, BrdU and pH3 triple-positive cell and arrowheads indicate Cd41-GFP and pH3 double-positive but BrdU-negative cell.