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Fig. S2 alcama and nadl1.1 ISH in edn1-/- mutants. (A, B) 30 hpf WT larvae showing expression of alcama and ednrb1 respectively. (C, D) alcama ISH showing expression in pharyngeal pouches of WT and edn1-/- larvae at 30 hpf. (E, F) 30 hpf larvae stained with Alcama antibody (Fast Red) and nadl1.1 RNA (Fluorescein). nadl1.1 expression in the NC is unchanged while Alcama is down-regulated in edn1-/- mutants (F). Arrows point to alcama expression in the pharyngeal pouches. Scale bars: 50 μm.
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Reprinted from Developmental Biology, 349(2), Choudhry, P., Joshi, D., Funke, B., and Trede, N., Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis, 483-493, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.