ZFIN Image: Choudhry et al., 2011, Fig. 7

Image

Figure Caption

Fig. 7 alcama and nadl1.1 interact during cartilage formation. (A) Lateral view of a 30 hpf WT larva stained with Zn-5 antibody (Fast Red) and anti-sense nadl1.1 RNA (Fluorescein), revealing that nadl1.1 is expressed in the NC adjacent to alcama in the endoderm. The bar graphs display the percentage of 30 hpf edn1^-/- (B) and WT (C) larvae with dlx5a staining after the indicated injections. (B) shows that nadl1.1 is required for alcama rescue of dlx5a expression in edn1^-/- mutants (p-value < 0.0001 by Fishers exact test). (C) and (D) show that alcama and nadl1.1 interact during dlx5a expression and cartilage morphogenesis. (D) Bar graph displaying the percentage of 5 dpf larvae with affected cartilage after the indicated injections (p-value < 0.0001 by χ² analysis). n indicates the number of experiments and N the total number of larvae. Scale bar: 50 μm.

Figure Data

Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 349(2), Choudhry, P., Joshi, D., Funke, B., and Trede, N., Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis, 483-493, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.