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Figure Caption
Fig. S2 Midline structures are intact in rock2b MO knockdown embryos. (A,E) Bright-field images of uninjected control (A) and rock2b MO (E) embryos, showing normal notochord formation. (B,C,F,G) RNA in situ hybridization analysis showed shh expression in the notochord and floorplate of the neural tube was similar in uninjected (n=71/71) (B,C) and rock2b MO (n=111/112) (F,G) embryos. (C,G) High-magnification views of the midline in B,F, respectively. (D,H) Expression of no tail (ntl) was contiguous in the notochords of uninjected (n=83/83) (D) and rock2b MO (n=86/87) (H) embryos.
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