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Fig. 2 rock2b is expressed in the DFC/KV cell lineage. (A,B) Whole embryo RNA in situ hybridization staining shows rock2b is expressed in DFCs (arrows) and the enveloping layer at 75% (A), 90% epiboly (B). (C,D) Sagittal sections of stained embryos at 95% epiboly show rock2b expression in DFCs (arrowhead) and the EVL (arrows). (E,F) During early SSs, rock2b is detected in KV cells (arrow). (E) is a lateral view at 1- to 2-SS and (F) is a dorsal view. (G-I) During later somitogenesis, rock2b is expressed in somites (arrows) and midline structures including the hypochord and notochord (arrowheads).
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