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Fig. 2 eng2a expression in pioneers requires maternal and/or residual lamc1, while ptc1 expression in the caudal part of the embryo is not affected in lamc1-depleted embryos. (A-F) Lateral views of eng2a expression at the 15 (A-C) and 20 s stage (D-F) in wild-type (A,D), sly (B,E) and morpholino-injected sly embryos (C,F). The fraction of embryos displaying the corresponding phenotype is shown on each panel. In 20 s stage sly embryos, no (n=3/11) to weak and transient expression (n=8/11) is found in only two or three caudal somites (red arrow in E). (G-I) Dorsal views of Laminin labelling (in red, blue arrows) at the tail bud (Tb) stage in wild-type (G), sly (H) and lamc1 MO-injected sly embryos (I). Dorsal (J-L) and lateral views (M-O) of ptc1 expression at the 15 s stage (J-L) and 24 hpf (M-O) in wild-type (J,M), sly (K,N,O) and lamc1 MO-injected sly embryos (L). Scale bar: 100 μm.