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Fig. S2 RT-PCR for DUOX Showing the efficiency of the DUOX splicing block morpholino. DUOX-morphant-typing primers amplify a 264-bp WT band from the cDNA of control, un-injected embryos, but embryos injected with DUOX morpholino exhibit exon escape, resulting in a 39-bp deletion of mRNA such that DUOX-morphant-typing primers amplify a smaller band from cDNA of DUOX morphant embryos. This knockdown is full at 3 dpf and retained until 5 dpf.
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